Embryo Biopsy
In short, the procedure is as follows::
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Day 3 post insemination embryo- |
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The embryo is held by suction to a micro-pipette, using micro-manipulators attached to the microscope. |
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A pipette is introduced to dissect a hole in the protein coat surrounding the embryo. |
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A cell is taken from the embryo using a biopsy pipette. |
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The cell containing the nucleus is released. |
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The cell is placed into a tube and treated for DNA analysis
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Day 3 post insemination embryo-
At the 8 cell stage
The embryo is held by suction to a micro-pipette, using micro-manipulators attached to the microscope.
A pipette is introduced to dissect a hole in the protein coat surrounding the embryo.
A cell is taken from the embryo using a biopsy pipette.
The cell containing the nucleus is released.
The cell is placed into a tube and treated for DNA analysis
