Kosmogonia IVF

Blastocyst grading system

embryoworks

The blastocyst grading system (Gardner)

	1. Blastocoel cavity less than half the volume of the blastocyst.
	2. Blastocyst cavity more than half the volume.
	3. Full Blastocyst-the blastocoel is filling the embryo.
	4. Expanded Blastocyst-the cavity is larger than the embryo and zona thinning occurs.
	5. Hatching Blastocyst-the trophectoderm/ outer layer is hatching out of its shell.
	6.Hatched Blastocyst. The blastocyst has completely hatched from the zona pellucida.
Inner cell mass quality (ICM): A. Many cells, tightly packed. B. Several cells, loosely packed. C. Few cells.	Trophectoderm quality: A. Many cells, forming a cohesive layer. B. Several cells, loose epithelium. C. Few cells.

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Embryo transfer.	Fertilisation-IVF.	Fertilised Oocyte.

The fertilized oocytes are cultured until the day of embryo transfer.	100,000 spermatozoa are added to each egg andincubated until the next morning.	The fertilised oocyte. showing two pronuclei. One from each parent