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How spermatozoa is prepared
Sperm recovery -How spermatozoa is prepared for IUI & IVF
Density gradient centrifugation ( formally known as the percoll gradient )
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1.A density gradient is made from silica-coated particles. Bottom layer 80%, top layer 40%. Semen is placed on top. |
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2.Centrifugation is used to separate the immotile debris from the motile spermatozoa. Motile spermatozoa are filtered out and accumulate at the bottom. |
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3.The sample is washed in culture media. |
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4.The most motile spermatozoa are counted and analyzed and used for I.U.I., insemination or ICSI. |  |
Swim up
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1.Test tube with culture media is placed at an angle of 45o. |
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2. After a period of time motile spermatozoa is aspirated from the top portion of the media after swimming up from the semen. | |
| 3. The most motile spermatozoa are counted and analyzed and used for insemination. | |
