Embryo Biopsy

In short, the procedure is as follows::

 

	Day 3 post insemination embryo- At the 8 cell stage
	The embryo is held by suction to a micro-pipette, using micro-manipulators attached to the microscope.
	A pipette is introduced to dissect a hole in the protein coat surrounding the embryo.
	A cell is taken from the embryo using a biopsy pipette.
	The cell containing the nucleus is released.
	The cell is placed into a tube and treated for DNA analysis

 

Day 3 post insemination embryo-
At the 8 cell stage

The embryo is held by suction to a micro-pipette, using micro-manipulators attached to the microscope.

 

A pipette is introduced to dissect a hole in the protein coat surrounding the embryo.

A cell is taken from the embryo using a biopsy pipette.

The cell containing the nucleus is released.

The cell is placed into a tube and treated for DNA analysis